UdemyGene Editing

Gene Editing Revolution: Molecular Biology, CRISPR Systems

التصنيف الكامل: Teaching & Academics > Science > Gene Editing

Gene Editing Revolution: Molecular Biology, CRISPR Systems

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It's an Unofficial Course.This course provides a comprehensive and structured exploration of modern gene editing, guiding learners from the fundamental principles of molecular biology to the most advanced CRISPR-based technologies used today. It begins by building a strong foundation in DNA structure, genetic inheritance, the genetic code, protein synthesis, and endogenous DNA repair mechanisms, ensuring that learners clearly understand how genetic information is stored, expressed, and maintained within living systems.As the course progresses, learners are introduced to the historical development of genetic engineering and the early tools that laid the groundwork for contemporary genome editing. Classical techniques such as restriction enzymes and recombinant DNA technology are explained in detail, followed by an in-depth examination of first-generation programmable nucleases, including zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs). These topics help learners appreciate the evolution of precision, efficiency, and specificity in genome manipulation.A major focus of the course is the CRISPR-Cas9 system, where learners gain a clear understanding of its biological origins, molecular components, and functional mechanisms. The roles of Cas proteins, guide RNA design, protospacer adjacent motifs, and double-strand break formation are thoroughly discussed. The course also explains how cellular repair pathways, such as non-homologous end joining and homology-directed repair, are harnessed to achieve targeted genetic modifications.Beyond conventional CRISPR-Cas9 editing, the course explores advanced and next-generation CRISPR technologies that enable greater precision and control. Learners will study base editing, prime editing, and CRISPR-based gene regulation systems such as CRISPR interference and CRISPR activation. Various gene delivery strategi

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